Çerez Örnek

Real-Time PCR

Real-time PCR

High-Sensitivity and High-Efficiency Molecular Analysis

Real-time PCR Device Image

Bio-Rad CFX96 Touch

Description

Bio-Rad CFX96 Touch Real-Time PCR System is a high-precision, high-efficiency real-time PCR (qPCR) instrument operating in a 96-well format. Fluorescence-based detection allows simultaneous monitoring of DNA and RNA amplification and enables quantitative analysis. With five different fluorescent channels, it supports multiplex analysis of multiple targets at once. The instrument is widely used in molecular biology applications such as gene expression analysis, pathogen detection, SNP genotyping, and DNA copy number determination, offering high sensitivity, a wide dynamic range, and user-friendly CFX Manager software.

Applications

  • Gene expression analysis (at mRNA level)
  • DNA and RNA quantification (Absolute or Relative Quantification)
  • Pathogen (Virus/Bacteria) detection and identification
  • Genotyping (SNP analysis)
  • Specificity check via melt curve analysis
  • CRISPR or genetic modification monitoring
  • miRNA or siRNA analysis
  • Viral load measurement (e.g., SARS-CoV-2, HBV)
  • DNA copy number determination (Copy Number Variation, CNV)
  • Comparative analysis of PCR data across multiple samples and time points

Device Hardware and Technical Specifications

Device Name: CFX96 Touch

  • Brand: Bio-Rad
  • Model: CFX Manager 96
  • 96-well real-time PCR instrument
  • Multiplex target analysis with five fluorescent channels
  • User-friendly CFX Manager software
  • High sensitivity and wide dynamic range
  • Simultaneous monitoring of DNA and RNA amplification
Real-time PCR qPCR Gene Expression Analysis Pathogen Detection

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Ege Üniversitesi

EGE UNIVERSITY