NanoDrop
NanoDrop
Micro-Volume UV-Vis Spectrophotometry
Thermo Scientific ND-8000
Description
The NanoDrop ND-8000 Micro-Volume UV-Vis Spectrophotometer enables rapid (<20 seconds) measurements of micro-volume samples (1 µL) across a wavelength range of 220–750 nm with a spectral resolution of ≤3 nm. Absorbance accuracy is ±3% at 0.74 Abs (350 nm), with a detection limit of 2.5 ng/µL for double-stranded DNA and 0.15 mg/mL for BSA. The instrument accommodates up to 8 samples and provides fast, reliable analysis for DNA, RNA, proteins, oligonucleotides, and more.
Applications
- DNA concentration and purity assessment (A260/A280, A260/A230)
- RNA concentration and purity assessment
- Protein concentration measurement (A280 or Bradford/BCA methods)
- Oligonucleotide quantification
- Lysate or cell extract analysis
- Tagged protein analysis (e.g., GFP, mCherry absorbance)
Device Hardware and Technical Specifications
Device Name: ND-8000
- Brand: Thermo Scientific
- Model: ND-8000
- Micro-volume UV-Vis spectrophotometer
- Wavelength range: 220–750 nm
- Spectral resolution: ≤3 nm (FWHM at Hg 546.1 nm)
- Absorbance range: 0–75 A
- Detection range: 2.5–3700 ng/µL dsDNA, 0.15–100 mg/mL BSA
- Sample volume: 1 µL
- Maximum samples: 8
NanoDrop UV-Vis Micro-Volume DNA/RNA/Protein Analysis
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