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NanoDrop

NanoDrop

Micro-Volume UV-Vis Spectrophotometry

NanoDrop Device Image

Thermo Scientific ND-8000

Description

NanoDrop Micro-Volume UV-Vis Spectrophotometer allows rapid (<20 seconds) measurements of micro-volume samples (1 µL) with a wavelength range of 220–750 nm and spectral resolution ≤3 nm. Absorbance accuracy is ±3% at 0.74 Abs (350 nm), with a detection limit of 2.5 ng/µL for dsDNA and 0.15 mg/mL for BSA. The instrument supports up to 8 samples and enables fast, reliable analysis for DNA, RNA, protein, and more.

Applications

  • DNA concentration and purity analysis (A260/A280, A260/A230)
  • RNA concentration and purity analysis
  • Protein concentration measurement (A280 or Bradford/BCA methods)
  • Oligonucleotide quantification
  • Lysate or cell extract analysis
  • Tagged protein analysis (e.g., GFP, mCherry absorbance)

Device Hardware and Technical Specifications

Device Name: ND-8000

  • Brand: Thermo Scientific
  • Model: ND-8000
  • Micro-volume UV-Vis spectrophotometer
  • Wavelength range: 220–750 nm
  • Spectral resolution: ≤3 nm (FWHM at Hg 546.1 nm)
  • Absorbance range: 0–75 A
  • Detection range: 2.5–3700 ng/µL dsDNA, 0.15–100 mg/mL BSA
  • Sample volume: 1 µL
  • Maximum samples: 8
NanoDrop UV-Vis Micro-Volume DNA/RNA/Protein Analysis

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